Our research deals with a major question in cell biology: how cell-selective transcription program, which is the basis for cell type specific functions, is progressively modulated during differentiation, faithfully transmitted through cell division, and attenuated in response to external stimuli. To address this question, we are studying three linked layers of transcription regulation: 

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• Transcription factor action

• Chromatin accessibility

• Three dimensional (3D) genome organization

 

We aim to reach an integrative view of how these three layers of regulation are orchestrating genome functions in various model organisms and biological processes.

 

To study these regulatory mechanisms we combine the power of next-generation sequencing with genome-wide approaches such as circular chromosome conformation capture followed (4C-seq), DNase hypersensitivity (DHS-seq), RNA sequencing (RNA-seq), Chromatin Immuno-precipitation (ChIP-seq) and Assay for Transposase-Accessible Chromatin (ATAC-seq) . We study the single-cell level using imaging approached such as 3D DNA FISH and immunofluorescence.